Detection of Class 1 Integron Encoding Gene in Multidrug Resistance (MDR) Citrobacter freundii Isolated from Healthy Broiler Chicken

This study was aimed to find out that broiler chicken farms have problems with antibiotic resistance Citrobacter freundii and determined the prevalence class 1 integron encoding gene. Multidrug collected from among one hundred sixty cloacal swab samples 32 in Blitar for 3 months. The method of bacterial inoculation used MacConkey agar biochemical test conducted by IMViC TSIA test. sensitivity pattern tested disk diffusion, multidrug gene PCR. exposed 160 samples, 13.75% (22/160) were positive freundii. showed high resistances against ampicillin erythromycin (77.27%), tetracycline (59.09%), trimetropim-sulfamethoxazole (50%), streptomycin (22.72%). Isolates detected as continued PCR testing prove existence a isolated 81.82% (18/22), indicated five Class Integron results this distribution high, so it can cause spread antimicrobial public health. found 22.72% It concluded need assessment management reduce avoid bacteria animals human. Therefore, use appropriate antibiotics is good step incidence MDR poultry.